What is a White Blood Cell Differential Count? Different types of leukocytes
leukocytes are formed through the process of leucopoiesis. There are primarily 2 types of white blood cells namely granular and agranular leukocytes. Granular leukocytes are aptly named so because these particular leukocytes contain conspicuous granules. Granular leukocytes are divided further into neutrophils, eosinophils and basophils; each containing granules with noticeable differences in colour among the three.
Agranular leukocytes, as the name suggests does not contain any granules. There are 2 types of agranular leukocytes mainly lymphocytes and monocytes. The B and T cells (lymphocytes) each have specific functions.
As mentioned earlier, granular leukocytes are distinctively different from agranular leukocytes due to the presence of granules. In addition to that, granular leukocytes have multi-lobed (2 or more lobes) nucleus whereas the nucleus of agranular leukocytes are bi-lobed.
Neutrophils being the most abundant of the 3 granular leukocytes (and apparently of the entire white blood cell population as well) constitutes 60-70% of the differential white blood cell count. Neutrophils stained pale lilac in acid-base dye. A cubic millimetre of blood has 3,000 to 7,000 counts of neutrophils. The cytoplasm stained pink and the multi-lobed (has 3 to 7 lobes) nucleus linked by thin strands of nucleoplasm, a deep purple. The granules are too fine to be seen under the microscope.
Eosinophils on the hand make up only 1-4% of the differential white blood cell count; a much smaller percentage compared to neutrophils. Eosinophils stained red-orange in acidic dye and blue-red for the bi-lobed nucleus. There are 100 to 400 counts of eosinophils per cubic millimetre of blood. Eosinophils are characterized by the presence of red coarse cytoplasmic granules.
Allergic reactions would trigger an increase in eosinophils number. Phagocytosis of parasitic worm occurs through the attachment of eosinophils onto the surface of the worm and destroys it (the worm is too large for eosinophils to engulf).
Basophils are leukocytes with the least differential white blood cell count, 0.25-5%. Basophils stained blue purple with basic dye and the nucleus shaped into a constricted S or U-shape stained dark blue. There are 20 to 50 counts of basophils per cubic millimetre of blood.
Upon inflammation, the blue-purple granules found in the cytoplasm are triggered to release histamine a vasolidator chemical to where it is required.
Lymphocytes and monocytes constitute only 25% of the white blood cells. Lymphocytes are the second most abundant leukocytes after neutrophils with 25-33% differential white blood cell count. A cubic millimetre of blood contains 1500 to 3000 counts of lymphocytes. The large nucleus stained dark purple and the thin layer of cytoplasm sky blue. The lymphocytes are mostly found lymph nodes, spleen and walls of large intestine in abundance.
Lymphocytes can be classified further into B and T lymphocytes. B lymphocytes engulf bacteria and prevent initiation of toxins. Antibodies which are microorganism-specific are produced to attack any foreign particles entering the body.
T lymphocytes are responsible for destroying any invading external agents. Rejection of foreign tissue can be associated with T lymphocytes. Thymus gland converts undeveloped cells to immunocompetent cells which are capable of performing such functions mentioned earlier.
Monocytes have a kidney-shaped nucleus which stained dark purple and the cytoplasm a grey-blue colour. Monocytes are different from lymphocytes in the way in which they are utilized. When an infection is detected, monocytes are mobilized and these leukocytes differentiate into macrophages. Once the macrophages are formed, phagocytosis of the foreign matter takes place. There is a surge in monocyte numbers during a persistent infection (one example would be tuberculosis).
Methodology of testing sample Conditions caused by Abnormal Counts of leukocytes
Infectious mononucleosis occurs when the number of lymphocytes in blood is abnormally high and structurally disrupted (not in the usual spherical form). Leukemia is caused by an uncontrollable sudden surge in numbers of leukocytes. The white blood cells count may reach as high as 50,000 cubic millimetre compared to the normal 7,000 cubic millimetre. Most of the cells are abnormal or immature thus preventing them from carrying out their normal defense function (quote). Leukemia affects the counts of other cells such as erythrocytes and platelets. Anaemia could result due to decreased production in red blood cells (or known as erythropoeisis). Insufficient platelet numbers causes internal bleeding. Both would eventually bring about a myriad of infections and haemorrhages. Patients suffering from leukemia most often die from these cases.
Body Processes of the Digestive System
Digestive system is made up of the digestive tract and other accessory organs. The purpose of digestive system is to convert food into small molecules that can be later on absorbed and utilized by the cells in the body. Food is broken down, until the molecules are minor enough to be absorbed and the waste products disposed. Alimentary canal or gastrointestinal (GI) tract are the different names for digestive tract, they consists of a long regular tube that start in the mouth, and goes down to the anus. The system includes the mouth, pharynx, esophagus, stomach, small intestine, and large intestine. The accessory structures that are present in the mouth are tongue and teeth. Salivary glands, liver, gallbladder, and pancreas are major accessory organ that play and important part in the process of digestion. These organs secrete fluids to the digestive tract. There are three types of processes that take place in the digestion system:
The two processes – digestion and absorption – take place in the digestive tract. After the process of absorption of the nutrients, they are ready for all cells in the body and then are utilized in metabolism. When the food that cannot be digested or absorbed by the body, it is eliminated. The unwanted waste products are in form of feces, which are called defecation or elimination.
Mouth – The digestive tract starts with mouth. When food enters mouth the chewing process breaks down food into smaller molecules for easier digestion, when saliva mixes with food the process of breaking down begins in the body, where everything starts to be absorbed and used in the body. Esophagus- Esophagus is located in the throat near trachea, the esophagus is receiving food from the mouth when it’s swallowed.
Stomach – The food enters the stomach through the passage of the cardiac sphincter. Food is further broken in the stomach, through a process of heuristic churning and is completely blended with digestive fluids, which is hydrochloric acid, and other digestive enzymes. The role of parietal cells of the stomach is to secrete a compound, which is an elemental factor in the vitamin B-12 absorption. When the level of acidity changes in the small intestines, more of the enzymes are being activated to break apart the structure of the specific nutrients so they can be absorbed into the circulatory or lymphatic systems. Small intestine – The role of small intestine is to absorb most of the nutrients found within digested food. By the time ingested food reaches the small intestine, it went through a process of mechanically broking down molecules into a liquid. As the liquid moves across the inner surface of the small intestine, nutrients from the food come into contact with the many small blood vessels which surround the small intestine. The inner surface of the small intestine has got a lot of small folds to increase the surface area. The blood exits the small intestine, carrying away all the necessary proteins and nutrients, water electrolytes, vitamins, minerals, fats and medications to the entire body.
Pancreas – The pancreas produce enzyme called insulin and pancreatic juice. Insulin is very important in human body as it controls sugar levels and also pancreatic juices contain enzymes that break up fats, proteins, and starch.
Liver – Liver produces bile, which is stored in the gallbladder until the small intestine needs it to break up large fat particles.
Gallbladder – The gallbladder stores the bile until it’s needed for digestion in the small intestines.
Colon (large intestine) – The function of large intestine is to absorb water from the remaining indigestible food, and then to pass useless and undigested waste material from the body.